If Itachi is the servant of evil who Len sings about? No. "Servant of Evil" is a song Len sings. It's the 秒 part of the "Story of Evil" series with the first being "Daughter of Evil". Len's character in the "Story of Evil" series is the servant to the 皇后乐队 (Played 由 Rin)
Plus, why the hell would the song be about Itachi? Len has no idea who Itachi is. He's not even an 日本动漫 character. He's a Vocaloid.
But if the role as "servant of evil" MATCHES Itachi is a different thing. There are small things that match. Such as Itachi loved Sasuke 更多 than anyone and anything, just like the servant of evil loved his twin sister - the daughter of evil (Yes, Len and Rin's characters are twins in the "Story of Evil" series).